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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is noticed in many tumors, which ends up in chromosome instability, and thus may well contribute to tumorigenesis. This protein was located to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a significant part in promoting cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, happen to be described. Two added splice variants ended up claimed but comprehensive nucleotide sequence details will not be nonetheless offered.
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Blocking peptides are peptides that bind specifically into the focus on antibody and block antibody binding. These peptide typically consists of the epitope regarded with the antibody.
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This information outlines more possible optimization of mobile and immunoassay parameters to make certain the best possible effects are...
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This guideline outlines even more probable optimization of mobile and immunoassay parameters to be certain the best possible final results are...
Antibodies sure to the blocking peptide now not bind to the epitope within the focus on protein. By evaluating the staining with the blocked antibody vs . the antibody by itself, you can see which staining is unique.
Target Information Cyclin E belongs towards the extremely conserved cyclin loved ones, whose associates are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S changeover. This protein accumulates in the G1-S section boundary and is also degraded as cells development by way of S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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